JPH0221794B2 - - Google Patents
Info
- Publication number
- JPH0221794B2 JPH0221794B2 JP56122815A JP12281581A JPH0221794B2 JP H0221794 B2 JPH0221794 B2 JP H0221794B2 JP 56122815 A JP56122815 A JP 56122815A JP 12281581 A JP12281581 A JP 12281581A JP H0221794 B2 JPH0221794 B2 JP H0221794B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- human
- cell line
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 85
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 claims description 23
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 claims description 15
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 claims description 15
- 230000002950 deficient Effects 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 229960005508 8-azaguanine Drugs 0.000 claims description 6
- WYWHKKSPHMUBEB-UHFFFAOYSA-N tioguanine Chemical compound N1C(N)=NC(=S)C2=C1N=CN2 WYWHKKSPHMUBEB-UHFFFAOYSA-N 0.000 claims description 4
- NKGPJODWTZCHGF-UHFFFAOYSA-N 9-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-3h-purine-6-thione Chemical compound OC1C(O)C(CO)OC1N1C(NC=NC2=S)=C2N=C1 NKGPJODWTZCHGF-UHFFFAOYSA-N 0.000 claims description 2
- 229960003087 tioguanine Drugs 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 140
- 239000002609 medium Substances 0.000 description 66
- 239000006228 supernatant Substances 0.000 description 30
- 230000007910 cell fusion Effects 0.000 description 23
- 210000004754 hybrid cell Anatomy 0.000 description 19
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 17
- 102000000588 Interleukin-2 Human genes 0.000 description 17
- 108010002350 Interleukin-2 Proteins 0.000 description 17
- 239000012894 fetal calf serum Substances 0.000 description 17
- 108010062580 Concanavalin A Proteins 0.000 description 16
- 238000000034 method Methods 0.000 description 16
- 239000012980 RPMI-1640 medium Substances 0.000 description 15
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 14
- 239000003795 chemical substances by application Substances 0.000 description 13
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 11
- 210000003719 b-lymphocyte Anatomy 0.000 description 11
- 230000000694 effects Effects 0.000 description 11
- 230000004927 fusion Effects 0.000 description 11
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 10
- 210000000349 chromosome Anatomy 0.000 description 9
- 230000035755 proliferation Effects 0.000 description 9
- 230000000638 stimulation Effects 0.000 description 9
- 239000012228 culture supernatant Substances 0.000 description 8
- 210000002741 palatine tonsil Anatomy 0.000 description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 210000004698 lymphocyte Anatomy 0.000 description 7
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 239000001569 carbon dioxide Substances 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 239000003226 mitogen Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 229940104230 thymidine Drugs 0.000 description 6
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 5
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 5
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 5
- 229960003896 aminopterin Drugs 0.000 description 5
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 5
- 210000005259 peripheral blood Anatomy 0.000 description 5
- 230000009257 reactivity Effects 0.000 description 5
- 102100028626 4-hydroxyphenylpyruvate dioxygenase Human genes 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 238000010166 immunofluorescence Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000000394 mitotic effect Effects 0.000 description 4
- 239000011886 peripheral blood Substances 0.000 description 4
- 229920001223 polyethylene glycol Polymers 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 102000008072 Lymphokines Human genes 0.000 description 3
- 108010074338 Lymphokines Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 241001494479 Pecora Species 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000002380 cytological effect Effects 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 210000000416 exudates and transudate Anatomy 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 230000002062 proliferating effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000002992 thymic effect Effects 0.000 description 3
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical group OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 102000005348 Neuraminidase Human genes 0.000 description 2
- 108010006232 Neuraminidase Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 208000002151 Pleural effusion Diseases 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 238000003163 cell fusion method Methods 0.000 description 2
- 239000002771 cell marker Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000007193 modulation by symbiont of host erythrocyte aggregation Effects 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
- APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 1
- 102000019260 B-Cell Antigen Receptors Human genes 0.000 description 1
- 108010012919 B-Cell Antigen Receptors Proteins 0.000 description 1
- 208000008720 Bone Marrow Neoplasms Diseases 0.000 description 1
- 108700031361 Brachyury Proteins 0.000 description 1
- 206010009152 Chronic tonsillitis Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000006354 HLA-DR Antigens Human genes 0.000 description 1
- 108010058597 HLA-DR Antigens Proteins 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 229930192392 Mitomycin Natural products 0.000 description 1
- 241000711408 Murine respirovirus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 108010092262 T-Cell Antigen Receptors Proteins 0.000 description 1
- 210000000662 T-lymphocyte subset Anatomy 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000061 acid fraction Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 1
- 229960003942 amphotericin b Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000006491 bone marrow cancer Diseases 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000007012 clinical effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 210000005104 human peripheral blood lymphocyte Anatomy 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 229940088592 immunologic factor Drugs 0.000 description 1
- 239000000367 immunologic factor Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000012121 regulation of immune response Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000012090 serum-supplement Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000007483 tonsillectomy Methods 0.000 description 1
- 206010044008 tonsillitis Diseases 0.000 description 1
- 229960001005 tuberculin Drugs 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (21)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56122815A JPS5823785A (ja) | 1981-08-04 | 1981-08-04 | ヒトｔ細胞ライン |
| US06/384,427 US4544632A (en) | 1981-06-12 | 1982-06-02 | Human T cell lines and method of producing same |
| NZ200849A NZ200849A (en) | 1981-06-12 | 1982-06-03 | Hypoxanthine-guanine-phosphoribosyl-transferase deficient human t leukemic cell line,and use in hybridoma technology |
| AU84474/82A AU555913B2 (en) | 1981-06-12 | 1982-06-04 | Hgprt deficient human t leukemic cell line |
| DK255882A DK255882A (da) | 1981-06-12 | 1982-06-07 | Humane t-cellelinier og fremgangsmaade til deres fremstilling |
| GB08216487A GB2102832B (en) | 1981-06-12 | 1982-06-07 | Human t cell lines and method of producing same |
| CA000404685A CA1189805A (en) | 1981-06-12 | 1982-06-08 | Human t cell lines and method of producing same |
| SE8203623D SE8203623L (sv) | 1981-06-12 | 1982-06-10 | Humana t-celler |
| FR8210121A FR2507620A1 (fr) | 1981-06-12 | 1982-06-10 | Lignees cellulaires t humaines et procede de production |
| SE8203623A SE460852B (sv) | 1981-06-12 | 1982-06-10 | T-cellinje dess framstaellning och anvaendning vid lymfokinframstaellning |
| CH3602/82A CH663795A5 (de) | 1981-06-12 | 1982-06-10 | Human-t-leukaemische zell-linie, hybrid-zell-linie, verfahren zur herstellung derselben und deren verwendung. |
| IT05179/82A IT1164454B (it) | 1981-06-12 | 1982-06-10 | Linee di cellule t umane e metodo per produrre le stesse |
| NL8202379A NL8202379A (nl) | 1981-06-12 | 1982-06-11 | Menselijke t-cellijnen en werkwijze voor het bereiden van deze cellijnen. |
| ES513027A ES8305039A1 (es) | 1981-06-12 | 1982-06-11 | "metodo de producir nuevas lineas de celulas t humanas". |
| DE3222072A DE3222072C2 (de) | 1981-06-12 | 1982-06-11 | Humane T-Zell-Linien und Verfahren zu ihrer Herstellung |
| DE3249567A DE3249567C2 (de) | 1981-06-12 | 1982-06-11 | Hybridzell-Linie, Verfahren zu ihrer Herstellung und ihre Verwendung |
| NO821949A NO162669C (no) | 1981-06-12 | 1982-06-11 | Fremgangsmaate for fremstilling av en hgprt-manglende human-t-leukemi cellelinje. |
| FI822103A FI76118C (fi) | 1981-06-12 | 1982-06-11 | Foerfarande foer framstaellning av en hgprt-defekt leukemisk human-t-cellinje. |
| AT0230482A AT387235B (de) | 1981-06-12 | 1982-06-14 | Menschliche t-zellen-linien und verfahren zu ihrer herstellung |
| AT0281883A AT377415B (de) | 1981-06-12 | 1983-08-04 | Stallbucht fuer zuchtsaeue |
| NO874129A NO162915C (no) | 1981-06-12 | 1987-10-01 | Fremgangsmaate for fremstilling av en human-t-hybrid-cellelinje. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56122815A JPS5823785A (ja) | 1981-08-04 | 1981-08-04 | ヒトｔ細胞ライン |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24962789A Division JPH02138969A (ja) | 1989-09-25 | 1989-09-25 | ヒトｔ交雑細胞ライン |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5823785A JPS5823785A (ja) | 1983-02-12 |
| JPH0221794B2 true JPH0221794B2 (en]) | 1990-05-16 |
Family
ID=14845317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56122815A Granted JPS5823785A (ja) | 1981-06-12 | 1981-08-04 | ヒトｔ細胞ライン |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5823785A (en]) |
-
1981
- 1981-08-04 JP JP56122815A patent/JPS5823785A/ja active Granted
Non-Patent Citations (1)
| Title |
|---|
| J BIOL CHEM * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5823785A (ja) | 1983-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5001065A (en) | Human cell line and triomas, antibodies, and transformants derived therefrom | |
| EP0043718A2 (en) | Improvements in or relating to cell lines | |
| JPH02227096A (ja) | 抗体およびその製法 | |
| JPS59169489A (ja) | ヒト培養株化細胞 | |
| MacLeish et al. | Use of a monoclonal antibody as a substrate for mature neurons in vitro. | |
| SK25596A3 (en) | Monoclonal antibody that is able to induce apoptosis, its fragments and hybridoma | |
| US4574116A (en) | Methods and cell lines for immortalization and monoclonal antibody production by antigen-stimulated B-lymphocytes | |
| Irigoyen et al. | Generation of functional human T cell hybrids. | |
| CA1189805A (en) | Human t cell lines and method of producing same | |
| Miller et al. | Cytoplasmic microtubules in transformed mouse× nontransformed human cell hybrids: Correlation with in vitro growth | |
| Schrader et al. | T cell hybridoma-derived regulatory factors. I. Production of T cell growth factor following stimulation by concanavalin A. | |
| Reimann et al. | Differentiation from precursors in athymic nude mouse bone marrow of unusual spontaneously cytolytic cells showing anti-self-H-2 specificity and bearing T cell markers. | |
| EP2009094B1 (en) | Fusion partner cells | |
| EP0107528B1 (en) | Human nonsecretory plasmacytoid cell line | |
| JPH0221795B2 (en]) | ||
| JPH0221794B2 (en]) | ||
| Fabricius et al. | Human primary T-cell lines in lectin-free media | |
| JPH02138969A (ja) | ヒトｔ交雑細胞ライン | |
| EP0077571A2 (en) | Process for producing a lymphokine | |
| CA1218947A (en) | Human hybrid cell lines | |
| JP2764101B2 (ja) | ヒトハイブリドーマ作製用の親細胞株 | |
| US4849507A (en) | Human suppressor factor | |
| JPH0551277B2 (en]) | ||
| JPH0351397B2 (en]) | ||
| Tseng et al. | Development and characterization of a human B cell line that responds to B cell growth factor but not interleukin 2. |